Membrane Proteomics
- Organelle isolation: differential velocity and equilibrium centrifugation methods are used to enrich for or purify specific cellular organelles.
- Protein identification: Proteins are separated by 1D or 2D –electrophoresis; peptides are separated using 2D- or 1D-liquid chromatography (LC); and proteins are identified by peptide mass fingerprinting and sequencing using MS/MS.
- Quantitative proteomics: fluorescent (2D-DIGETM) or isotopic labelling of proteins or peptides (I-TRAQTM, ICATTM, SILACTM) is used to determine the relative abundance of proteins.
| Last modified: | 03 October 2012 12.04 p.m. |