PhD defence H. Guo

Development of novel molecules to study lipoxygenase activity in its cellular context

15-lipoxygenase-1 (15-LOX-1) is an enzyme involved in the biosynthesis of inflammatory signaling molecules having key regulatory roles in immune responses and numerous diseases, such as asthma, COPD, atherogenesis, diabetes, stroke, Alzheimer’s disease and Parkinson’s disease, as well as cancer. For millions of patients suffering from these diseases the therapeutic possibilities are limited. Hence, addressing these unmet clinical needs is urgently needed. Therefore, Hao Guo aimed to develop small molecular inhibitor and chemistry-based detection method in the LOX field further in order to gain more knowledge and expand the therapeutic possibilities for these inflammatory diseases. In this study, he presented the development of novel 15-LOX-1 inhibitor and their biological evaluation in cell-based studies. Furthermore, he also presented the development of novel 15-LOX-1 inhibitors in combination with the development of novel probes for activity-based detection of 15-LOX-1 activity. In this way, Guo aimed to develop of inhibitors for which one can prove LOX inhibition in its cellular context.

Promotores: Prof.dr. F.J. Dekker and Prof.dr. H.J. Haisma