Masterclass "Computational Approaches and In Silico Enzyme Library Design for Applied Biocatalysis"

July 1-5, 2019
University of Groningen
Linnaeusborg & Bernoulliborg, Nijenborgh 7 & 9, 9747 AG Groningen
The Netherlands

Goal and outline

The course aims to make participants familiar with the use of structural biology and computational tools for engineering the performance of enzymes that are relevant for applied biocatalysis and synthetic biology. The course provides both a theoretical background on computational methods relevant to enzyme engineering (protein crystallography, homology modeling, energy calculations, protein design, smart libraries), and hands-on computer exercises on visualization and analysis of enzymes structures, including docking simulations and enzyme redesign. The theoretical and practical parts are integrated in a few problem-solving modules. Participants will have the opportunity to share and discuss their research via short oral presentations, posters, and interactive lectures.

Topics

• Protein crystallography and structure analysis
• Homology modeling docking and molecular dynamics simulations (Yasara)
• Computational enzyme (re)design (Rosetta)
• Enzyme engineering supported by web tools
• Sequence- and structure-based smart libraries
• Thermostability and stereoselectivity engineering
• Industrial examples of enzyme engineering

Tutors and teachers (to be expanded)

Dick Janssen, University of Groningen
Hein Wijma, University of Groningen
Andy-Mark Thunnissen, University of Groningen
Jiri Damborsky, Masaryk University, Brno
David Bednar, Masaryk University, Brno
Emanuele Monza, Zymvol, Barcelona
Marc van der Kamp, University of Bristol, United Kingdom
Rene de Jong, The Netherlands
t.b.a.

Masterclass coordination

Dick Janssen, GBB, Hein Wijma and Marco Fraaije, University of Groningen, The Netherlands
Jiri Damborsky, David Bednar, Masaryk University, Brno, Czech Republic
Sandra Haan & Tamara Hummel, GBB, University of Groningen, The Netherlands

Intended participants

PhD students, post docs and other researchers skilled in biochemistry who want to become familiar with structure-based and computational approaches in Enzyme Engineering. Partners of the H2020 ES-Cat and other EU collaborative projects and training networks are offered priority reduced fee registration.

Note: participants are offered the opportunity to share their research via posters and participant talks.